Identification of five sea cucumber species through PCR-RFLP analysis     

Yingchun Lv  Rong Zheng  Tao Zuo  Yuming Wang  Zhaojie Li  Yong Xue  Changhu Xue  Qingjuan Tang 《中国海洋大学学报(英文版)》2014,13(5):825-829

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.  相似文献   

Field experiments of multi-channel oceanographic fluorescence lidar for oil spill and chlorophyll-a detection     

Xiaolong Li  Chaofang Zhao  Youjun Ma  Zhishen Liu 《中国海洋大学学报(英文版)》2014,13(4):597-603

A Multi-channel Oceanographic Fluorescence Lidar (MOFL), with a UV excitation at 355 nm and multiple receiving channels at typical wavelengths of fluorescence from oil spills and chlorophyll-a (Chl-a), has been developed using the Laser-induced Fluorescence (LIF) technique. The sketch of the MOFL system equipped with a compact multi-channel photomultiplier tube (MPMT) is introduced in the paper. The methods of differentiating the oil fluorescence from the background water fluorescence and evaluating the Chl-a concentration are described. Two field experiments were carried out to investigate the field performance of the system, i.e., an experiment in coastal areas for oil pollution detection and an experiment over the Yellow Sea for Chl-a monitoring. In the coastal experiment, several oil samples and other fluorescence substances were used to analyze the fluorescence spectral characteristics for oil identification, and to estimate the thickness of oil films at the water surface. The experiment shows that both the spectral shape of fluorescence induced from surface water and the intensity ratio of two channels (I ₄₉₅/I ₄₀₅) are essential to determine oil-spill occurrence. In the airborne experiment, MOFL was applied to measure relative Chl-a concentrations in the upper layer of the ocean. A comparison of relative Chl-a concentration measurements by MOFL and the Moderate Resolution Imaging Spectroradiometer (MODIS) indicates that the two datasets are in good agreement. The results show that the MOFL system is capable of monitoring oil spills and Chl-a in the upper layer of ocean water.  相似文献   

湛江地区一次冷锋型海雾微物理特征   总被引：1，自引：1，他引：0       下载免费PDF全文

吕晶晶  牛生杰  赵丽娟  张羽  徐峰 《大气科学学报》2014,37(2):208-215

利用2010年3月31日—4月2日冷锋天气系统影响下湛江海雾综合观测资料,分析了海雾的微物理特征及海雾过程中气溶胶粒子谱的演变特征。结果表明,海雾的生消与风场密切相关,海雾生成和发展与较强的ESE气流相联系,而弱的NE气流则会促使海雾减弱或消散。湛江海雾的雾滴数浓度为100~102cm-3,液态含水量为0.001~0.232 g·m-3,雾滴平均半径小于10μm,雾滴峰值半径多位于1.4μm。海雾雾滴谱分布以单调递减谱为主,谱宽超过20μm,且雾发展过程中雾滴谱谱宽存在突然增宽和迅速减小的现象。海雾过程中雾滴数浓度的变化主要是由半径小于5μm的雾滴数密度变化引起的。海雾过程对气溶胶粒子的湿清除效果并不显著,雾过程中粒径小于0.1μm和大于4μm的气溶胶粒子数密度显著减少,但在雾消散后又迅速恢复到雾发生前的水平。  相似文献   

Effects of the oophagous bivalve Acesta oophaga on the morphology and fecundity of its deep‐sea tubeworm host,Lamellibrachia luymesi     

Zair P. Burris  Joshua P. Lord  Craig M. Young 《Marine Ecology》2014,35(1):106-111

The deep‐sea bivalve Acesta oophaga lives attached to the anterior end of the vestimentiferan tubeworm, Lamellibrachia luymesi, at cold methane seeps. The bivalve is found almost exclusively on female tubeworms, where it consumes the lipid‐rich eggs of L. luymesi that are spawned year round (Biological Bulletin, 209, 2005, 87). It is apparent that A. oophaga benefits directly from this close association, but the consequences for the tubeworm host may be more complicated than just a simple predator–prey interaction. Since A. oophaga completely surrounds the tube opening and plume of the worm, it is likely that its presence would limit oxygen uptake by L. luymesi, thereby inhibiting worm growth and reproduction. We hypothesized that occupied tubeworms would compensate for this by growing larger plumes for oxygen uptake. To explore the effects of bivalve presence/absence on female tubeworms, several morphological features, including body size, plume length, tube diameter, and tube segment length, as well as instantaneous fecundity, were compared. Results suggest that the mere presence of A. oophaga has a significant impact on the morphology of its host worm, as all measures of worm size, except for tube segment length, were significantly greater with clams present. Additionally, instantaneous fecundity was 3.5 times higher in occupied worms, implying that tubeworms are not oxygen‐deprived or energy limited as a result of bivalve presence. Our findings suggest that the association between these two deep‐sea organisms may be a more complex form of symbiosis than the simple predator–prey relationship, as previously thought.  相似文献   

一次典型高压型海雾过程中海上大气波导的数值模拟     

袁夏玉  高山红 《海洋与湖沼》2014,45(5):907-917

2009年4月9—12日黄海海域发生了一次受高压系统影响的海雾过程。利用卫星观测与探空数据、WRF模式(Weather Research and Forecasting Model)对此次海雾过程及相伴的大气波导进行了观测分析与数值模拟。海雾与波导发展可分为3个阶段:(1)大气波导先于海雾存在于黄海海面;受高压下沉影响,黄海上空存在逆温层和较强的湿度梯度,表现为较强的贴海表面波导和非贴海表面波导。(2)海雾始于高压西部,并随高压系统逐渐东移减弱,向黄海北部扩展;辐射冷却虽然使雾顶附近逆温增强,但海雾的机械湍流使其顶部湿度梯度减小,雾顶附近对应弱悬空波导或波导消失。(3)高压系统影响使干空气下沉到雾区导致黄海海雾消散;雾顶附近逆温仍存在,同时湿度梯度增大,黄海上空逐渐变为非贴海表面波导。本研究结果表明:高压系统不仅极易为波导的发生提供有利条件,而且有利于海雾的生成,在海雾演变过程中主要是雾顶水汽梯度的变化导致了波导类型及强度的变化。  相似文献   

基于ROMS模式利用集合最优插值同化沿轨海表面高度异常数据的研究   总被引：2，自引：1，他引：1  

LYU Guokun  WANG Hui  ZHU Jiang  WANG Dakui  XIE Jiping  LIU Guimei 《海洋学报(英文版)》2014,33(7):72-82

The ensemble optimal interpolation （EnOI） is applied to the regional ocean modeling system （ROMS） with the ability to assimilate the along-track sea level anomaly （TSLA）. This system is tested with an eddy-resolving system of the South China Sea （SCS）. Background errors are derived from a running seasonal ensemble to account for the seasonal variability within the SCS. A fifth-order localization function with a 250 km localization radius is chosen to reduce the negative effects of sampling errors. The data assimilation system is tested from January 2004 to December 2006. The results show that the root mean square deviation （RMSD） of the sea level anomaly decreased from 10.57 to 6.70 cm, which represents a 36.6% reduction of error. The data assimilation reduces error for temperature within the upper 800 m and for salinity within the upper 200 m, although error degrades slightly at deeper depths. Surface currents are in better agreement with trajectories of surface drifters after data assimilation. The variance of sea level improves significantly in terms of both the amplitude and position of the strong and weak variance regions after assimilating TSLA. Results with AGE error （AGE） perform better than no AGE error （NoAGE） when considering the improvements of the temperature and the salinity. Furthermore, reasons for the extremely strong variability in the northern SCS in high resolution models are investigated. The results demonstrate that the strong variability of sea level in the high resolution model is caused by an extremely strong Kuroshio intrusion. Therefore, it is demonstrated that it is necessary to assimilate the TSLA in order to better simulate the SCS with high resolution models.  相似文献   

Amodified discrete element model for sea ice dynamics     

LI Baohui  LI Hai  LIU Yu  WANG Anliang  JI Shunying 《海洋学报(英文版)》2014,33(1):56-63

Considering the discontinuous characteristics of sea ice on various scales,a modified discrete element model（DEM） for sea ice dynamics is developed based on the granular material rheology.In this modified DEM,a soft sea ice particle element is introduced as a self-adjustive particle size function.Each ice particle can be treated as an assembly of ice floes,with its concentration and thickness changing to variable sizes under the conservation of mass.In this model,the contact forces among ice particles are calculated using a viscous-elastic-plastic model,while the maximum shear forces are described with the Mohr-Coulomb friction law.With this modified DEM,the ice flow dynamics is simulated under the drags of wind and current in a channel of various widths.The thicknesses,concentrations and velocities of ice particles are obtained,and then reasonable dynamic process is analyzed.The sea ice dynamic process is also simulated in a vortex wind field.Taking the influence of thermodynamics into account,this modified DEM will be improved in the future work.  相似文献   

基于阈值区间的海洋锋面提取模型   总被引：1，自引：0，他引：1  

PING Bo  SU Fenzhen  MENG Yunshan  FANG Shenghui  DU Yunyan 《海洋学报(英文版)》2014,33(7):65-71

A model （Bayesian oceanic front detection, BOFD） of sea surface temperature （SST） front detection in satel- lite-derived SST images based on a threshold interval is presented, to be used in different applications such as climatic and environmental studies or fisheries. The model first computes the SST gradient by using a Sobel algorithm template. On the basis of the gradient value, the threshold interval is determined by a gradi- ent cumulative histogram. According to this threshold interval, front candidates can be acquired and prior probability and likelihood can be calculated. Whether or not the candidates are front points can be deter- mined by using the Bayesian decision theory. The model is evaluated on the Advanced Very High-Resolution Radiometer images of part of the Kuroshio front region. Results are compared with those obtained by using several SST front detection methods proposed in the literature. This comparison shows that the BOFD not only suppresses noise and small-scale fronts, but also retains continuous fronts.  相似文献   

应用极化合成孔径雷达检测海上溢油研究进展   总被引：4，自引：2，他引：2  

邹亚荣  邹斌  梁超 《海洋学报》2014,36(9):1-6

海上溢油给海洋生态环境带来严重的影响,快速准确地探测溢油对于防灾减灾具有重要的意义。利用卫星遥感探测溢油已成为目前主要的检测手段,大多采用合成孔径雷达(SAR)数据,运用图像处理的方法,开展了多种溢油提取算法的研究,取得了较好的结果,但由于海洋的类溢油现象存在,造成提取信息的精度达不到要求。近年来,国内外运用极化SAR数据开展溢油信息提取研究,从极化分解与相位差等角度对溢油特性分析,能有效地区分一些类溢油现象,得到了较理想的结果。分析了应用SAR数据开展溢油信息提取的研究状况,总结了溢油极化SAR探测的研究,指出了目前研究中存在的不足,并提出了今后溢油极化SAR遥感监测的方向。  相似文献   

中国近海海平面变化与ENSO的关系   总被引：2，自引：1，他引：1  

王慧  刘克修  张琪  范文静  高志刚  张增建  李琰 《海洋学报》2014,36(9):65-74

利用中国沿海台站潮位和中国近海及赤道太平洋的卫星测高、海表温度、风及气压资料,分析了中国近海海平面变化与ENSO的关系。分析结果表明:中国沿海海平面季节变化受ENSO影响明显,在厄尔尼诺事件期间,中国沿海海平面Sa分潮的振幅明显减小,其中年振幅的历史极小值均出现厄尔尼诺年,不同区域历史极小值出现的年份不同;另外,中国沿海Sa分潮的振幅对厄尔尼诺事件的响应与其强弱有关,在强事件中,响应区域和幅度较大,弱事件中,响应区域和幅度偏小。在厄尔尼诺年,中国沿海海平面多低于相邻年份,并且其年际变化存在明显的2~3a、4~7a、准9a、11a和准19a的周期,其中4~7a的周期在冬春季节震荡最显著,其震荡幅度接近2cm。中国近海海平面与赤道东太平洋区域的海表温度年际变化之间存在反相关关系,其相关系数为-0.42;同时与Nio4和Nio3.4指数序列也呈现反相关关系。针对典型的1997/1998年尼诺事件发生前后的风场和气压场分析发现,尼诺发生前的冬半年,冬季风偏强,气压梯度加强,中国沿海海平面偏低;到了厄尔尼诺的盛期,出现较强的南风异常,气压梯度反向,季风转向,过渡到了厄尔尼诺事件的衰减期,为拉尼娜事件做准备,此时海平面偏高。  相似文献